16 April 2003

Biotech - No panacea/New push for patents on life/Spectre orange

"If sophisticated instrumentation and fatly-paid scientific staff were the answers, [India] would not have seen the "Green Revolution" fall on its face, with degraded soils, drying aquifers and vanishing biodiversity. More than 20 per cent of the soil of Punjab and Haryana, the cradle of the Green Revolution, is beyond repair, where it will be a Herculean task to grow even a blade of grass again! The process fattened
the farm lobby while the less-than-one-acre poor farmer was left out in the cold. No Green Revolution ever happened on his tiny patch of land.

The country seems headed on a similar course, where profit is the driving force rather than public good. When will we learn to place life firmly before profit?" - K. P. Prabhakaran Nair [item 2 - very interesting article]

"One floor below, it is never quiet. Here are those who have survived the misery of their births, ravaged infants whom no one has the ability to understand, babies so traumatised by their own disabilities, luckless children so enraged and depressed at their miserable fate, that they are tied to their beds just to keep them safe from harm." - item 3

"There is huge pressure at the moment from the industry on government to basically reverse the decision of the Supreme Court legislatively" -item 1

*Biotech industry seeks legislation to permit patenting of life
*Biotech: No panacea for farm sector's ills
*[Monsanto's] Spectre orange
Biotech industry seeks legislation to permit patenting of life
Canadian Press

OTTAWA - Canada's biotech industry says its prospects have been hard hit by the Supreme Court decision against patenting the Harvard mouse.

Industry spokesmen said Monday the ruling has had a chilling effect on foreign investment and called on Ottawa to introduce legislation to permit patenting higher life forms.

But ethicists and environmentalists said the court had it right when it refused to approve a patent on the genetically altered mouse intended for use in cancer research.

The Dec. 5 decision, which stunned many in the biotech industry, makes Canada the only major industrial country that prohibits patenting life forms.

"This decision has sent an extremely negative signal to the global biotech and financial communities," said Bertrand Bolduc of TGN Biotech in Quebec City at a debate sponsored by the Intellectual Property Institute of Canada.

He said his company has had difficulty raising funds abroad because investors are puzzled by the ruling.

Patent lawyer David Morrow said there's no reason for Canada to have a more limited definition of what is patentable than its trading partners and competitors.

Mr. Morrow said the court's ruling was not based on environmental or policy considerations, but on the fact that genetic engineering was not forseen when the Patent Act was written.

He called for the act to be revised to ensure the patentability of plants, non-human animals and micro-organisms.

Michelle Swenarchuk, a lawyer with the Canadian Environmental Law Association, disputed the claim by industry that patents stimulate innovation, saying they sometimes have the opposite effect.

She said the provinces are worried about financial pressure on the health system due to the growing number of patented genetic tests. She cited the case of Myriad Genetics, a U.S. firm which has patented two genes implicated in breast cancer and claims a monopoly on all tests based on those genes.

The Ontario government is refusing to recognize the Myriad claim and is using cheaper alternatives. Ms. Swenarchuk said other provinces are lining up with Ontario on the issue.

Eric Beresford, a consultant to the Canadian Council of Churches, said life is not a human invention and therefore should not be patentable.

"There is huge pressure at the moment from the industry on government to basically reverse the decision of the Supreme Court legislatively," said Mr. Beresford.

Biotech: No panacea for farm sector's ills
K. P. Prabhakaran Nair
The Hindu Business Line, India, 15/04/2003

NOT long ago a Chennai-based privately funded Foundation made what was called the "Chennai Declaration": that there is an imminent need for the public acceptance of
biotechnology. A little before this, the newly appointed Director General of the Indian Council of Agricultural Research (ICAR), endorsed the need for the country to take the "biotechnology route" to enhance crop production, during his first press meet. And, to cap it all, Dr Craig Venter, the "author-discoverer" of the human genome,
heralding the "genome revolution", was in New Delhi recently to attend the "Knowledge Millennium III: The Business of Biotechnology" organised by Assocham, and to tell Indians that biotechnology is "the need of the hour".

 India is thus being hailed as the next destination, after the US, to see all the "biotech action", and there is talk of "limitless riches", as someone colourfully put it, in the making. Indeed, Indian farmers are being told that their salvation lies in biotechnology. This article is not meant to repudiate or endorse what is increasingly being heard on biotechnology being the panacea for the ills of Indian agriculture, but to make the plea that before we take a reckless plunge, it might be educative to pause and ponder. Not long ago the country was witness to the hysteria over the "Bollgard" cotton, peddled by the MNC Monsanto and its native collaborator Mahyco. The Genetic Engineering Approval Committee (GEAC), supported by the ICAR, cleared the controversial cotton on March 26, 2002.

 When the Bollgard cotton was cleared for commercial cultivation in some parts of India, this author had cautioned against the move ("Bt cotton: Bane or Boon?", Business Line, April 9, 2002) and outlined a strategy for follow-up monitoring. Nearly 50,000 farmers had sown the seeds, covering close to 40,000 hectares. Notwithstanding Mahyco's claims to the contrary, several farmers felt let down by the crop's performance and sued the Maharashtra government and the Monsanto Corporation for Rs 500 crore in damage. A packet of Bollgard cotton was priced at Rs 1,600 for 450 gm, and the Mahayco-Monsanto Biotech Ltd (MMB), the50:50 joint venture, walked away with Rs 16.8 crore from sale of seeds. At the time the Bollgard seed was touted as the wonder solution for cotton farmers distraught with the Bollworm menace, the MNC had claimed "superior" yield as a bonus, in addition to resistance to the insect pest.

 Interestingly, these claims have been belied, and whenever the crop failed, it was attributed to the "adverse" weather conditions. The most startling evidence of the failure of Bt cotton comes from a report commissioned by the GEAC, following widespread complaints of sudden failure of the crop in the Mandaleshwar block in Khargone district in Madhya Pradesh. GEAC sent a seven-member team to investigate the crop failure. The team comprised a cotton physiologist, cotton pathologist, cotton agronomist, cotton soil scientist, cotton entomologist and a cotton plant breeder - the full complement of cotton sciences - and it reported large-scale wilting and drying of the plant at the peak boll formation stage, accompanied by drooping leaves, leaf shedding and bursting of bolls. The "refuge" plants of cotton (non Bt variety) planted on the borders of the Bt cotton were far less affected. The possibility of a soil fungus Fusarium or some soil nutrient deficiency as the cause for the wilting was ruled out through controlled laboratory experiments. The scientific team pointedly suggested in its report that "a genetically controlled physiological disorder" might have triggered the widespread wilting and the drought was not the culprit as the MNC was trying to make out. Here, there is a parallel between what conventional plant breeders achieved more than four decades ago - which ushered in the so-called "Green Revolution" - through classical hybridisation process, where intra-specific crosses were made and the resultant "miracle" dwarf varieties of wheat and rice were obtained.

 The main difference between what the classical plant breeders of the 1960s did and what the genetic engineers are trying to do now is that while the former looked at the entire plant, the latter are only looking at a single gene, that could reportedly possess a specific trait. Even in the latter, the newly-bred plant variety might break down in respect of its acquired trait over the years. Instances are many of the new miracle varieties of wheat and rice succumbing to pests and diseases. This shows that what the classical plant breeders did and what the new breed of genetic engineers are doing are both at the periphery of our true understanding of what, in simple terms, could be called "life". But two crucial things remain unsaid. First, none of the agencies engaged in evolving the genetically engineered crops bother to provide information on the biochemical activity of the alien gene, either of animal origin, as in the case of the Bt cotton - where the Bacillus thuringiensis (Bt), a naturally occurring bacterium in soil that produces the organic toxin, was utilised - or of plant origin, as in the case of "Roundup Ready" wheat that Monsanto is readying for commercial cultivation. Such information would have helped track unexpected impacts, as has been observed with the sudden collapse of the Bt cotton in many farmers' fields in India or the transformation of the entire genome of the transgenic Monsanto soybean, resulting in potentially harmful proteins after inserting an alien gene, as observed recently in a case study in Belgium. There are other reports of such abnormal transformations taking place in transgenic rice leading to unpredictable consequences.

Admittedly, there are disturbing lacunae in the understanding of genetic engineering, because those involved in the enterprise have an over-simplistic view of the centrality of molecular biology. I wish to add, with humility, that those involved in the genetic engineering race are far too ill-equipped to strike at the root of understanding, because that is a far more demanding academic task, than merely isolating and inserting a gene from an alien organism, either a bacterium or plant, into a different host. The abnormal field variations that we observe in the transgenic plant can all be traced to the little understood and far too little explored ribonucleic acid (RNA) based networks of gene regulation. These are critical to our true understanding of these phenotypic observations in the field, as in the case of plants, or instances of enlarged hearts, gastric ulcers, arthritis and renal disease in gene-modified pigs or the most recent instance of a child, being treated with gene therapy for the Severe Combined Immunodeficiency Disease (SCID), developing leukaemia (Nature, December 2002).

What does all this show? That we must abandon biotechnological research? Far from it. On balance, if properly directed, the benefits might far outweigh the risks. But there are "ifs'. Indeed, very large `ifs'. If only our scientists equip themselves with superlative technical skills, backed by unimpeachable academic credentials and admirably motivated by visionary leadership and supported with almost limitless funding! Sophisticated instrumentation and an army of "armchair" scientists is no answer to unravelling the true story of the gene. Within the developing world, India has the largest public-funded R&D establishment, with only China and Brazil showing comparable levels of expenditure and staff size, but with much to their credit than we have achieved! Colossal amounts are being spent by the Department of Biotechnology, the monolith ICAR, 31 agricultural universities spread across the length and breadth of this country (some engaged in cotton "research" as well), 81 national research centres (some of which also do research on cotton), the apex Central Cotton Research Institute (doing exclusive research on cotton), the endless commissions on agriculture (the most recent being the National Commission on Agriculture); Parliamentary panels, "brainstorming" by the "super scientist turned administrators", Planning Commission meetings, and so on.

Despite all this, why could India not dream up a Bt cotton of its own, which could have, by hindsight, pre-empted the American MNCs' onslaught on Indian soil? All these are very uncomfortable questions for which we have no answers. If sophisticated instrumentation and fatly-paid scientific staff were the answers, the country would not have seen the "Green Revolution" fall on its face, with degraded soils, drying aquifers and vanishing biodiversity. More than 20 per cent of the soil of Punjab and Haryana, the cradle of the Green Revolution, is beyond repair, where it will be a Herculean task to grow even a blade of grass again! The process fattened the farm lobby while the less-than-one-acre poor farmer was left out in the cold. No Green Revolution ever happened on his tiny patch of land.

 The country seems headed on a similar course, where profit is the driving force rather than public good. When will we learn to place life firmly before profit?

The author is a senior fellow of the Alexander von Humboldt Foundation. The Alexander von Humboldt Foundation is a non-profit foundation established by the Federal Republic of Germany for the promotion of international research cooperation.
Full details at]

Copyright (c) 2003, The Hindu Business Line
Spectre orange
By Cathy Scott-Clark and Adrian Levy
The Guardian, Saturday March 29, 2003,3605,923715,00.html

Hong Hanh is falling to pieces. She has been poisoned by the most toxic molecule known to science; it was sprayed during a prolonged military campaign. The contamination persists. No redress has been offered, no compensation. The superpower that spread the toxin has done nothing to combat the medical and environmental catastrophe that is overwhelming her country. This is not northern Iraq, where Saddam Hussein gassed 5,000 Kurds in 1988. Nor the trenches of first world war France. Hong Hanh's story, and that of many more like her, is quietly unfolding in Vietnam today. Her declining half-life is spent unseen, in her home, an unremarkable concrete box in Ho Chi Minh City, filled with photographs, family plaques and yellow enamel stars, a place where the best is made of the worst.

Hong Hanh is both surprising and terrifying. Here is a 19-year-old who lives in a 10-year-old's body. She clatters around with disjointed spidery strides which leave her soaked in sweat. When she cannot stop crying, soothing creams and iodine are rubbed into her back, which is a lunar collage of septic blisters and scabs. "My daughter is dying," her mother says. "My youngest daughter is 11 and she has the same symptoms. What should we do? Their fingers and toes stick together before they drop off. Their hands wear down to stumps. Every day they lose a little more skin. And this is not leprosy. The doctors say it is connected to American chemical weapons we were exposed to during the Vietnam war."

There are an estimated 650,000 like Hong Hanh in Vietnam, suffering from an array of baffling chronic conditions. Another 500,000 have already died. The thread that weaves through all their case histories is defoliants deployed by the US military during the war. Some of the victims are veterans who were doused in these chemicals during the war, others are farmers who lived off land that was sprayed. The second generation are the sons and daughters of war veterans, or children born to parents who lived on contaminated land. Now there is a third generation, the grandchildren of the war and its victims.

This is a chain of events bitterly denied by the US government. Millions of litres of defoliants such as Agent Orange were dropped on Vietnam, but US government scientists claimed that these chemicals were harmless to humans and short-lived in the environment. US strategists argue that Agent Orange was a prototype smart weapon, a benign tactical herbicide that saved many hundreds of thousands of American lives by denying the North Vietnamese army the jungle cover that allowed it ruthlessly to strike and feint.

New scientific research, however, confirms what the Vietnamese have been claiming for years. It also portrays the US government as one that has illicitly used weapons of mass destruction, stymied all independent efforts to assess the impact of their deployment, failed to acknowledge cold, hard evidence of maiming and slaughter, and pursued a policy of evasion and deception.

Teams of international scientists working in Vietnam have now discovered that Agent Orange contains one of the most virulent poisons known to man, a strain of dioxin called TCCD which, 28 years after the fighting ended, remains in the soil, continuing to destroy the lives of those exposed to it. Evidence has also emerged that the US government not only knew that Agent Orange was contaminated, but was fully aware of the killing power of its contaminant dioxin, and yet still continued to use the herbicide in Vietnam for 10 years of the war and in concentrations that exceeded its own guidelines by 25 times. As well as spraying the North Vietnamese, the US doused its own troops stationed in the jungle, rather than lose tactical advantage by having them withdraw.

On February 5, addressing the UN Security Council, secretary of state Colin Powell, now famously, clutched between his fingers a tiny phial representing concentrated anthrax spores, enough to kill thousands, and only a tiny fraction of the amount he said Saddam Hussein had at his disposal. The Vietnamese government has its own symbolic phial that it, too, flourishes, in scientific conferences that get little publicity. It contains 80g of TCCD, just enough of the super-toxin contained in Agent Orange to fill a child-size talcum powder container. If dropped into the water supply of a city the size of New York, it would kill the entire population. Ground-breaking research by Dr Arthur H Westing, former director of the UN Environment Programme, a leading authority on Agent Orange, reveals that the US sprayed 170kg of it over Vietnam.

John F Kennedy's presidential victory in 1961 was propelled by an image of the New Frontier. He called on Americans to "bear the burden of a long twilight struggle ... against the common enemies of man: tyranny, poverty, disease, and war itself." But one of the most problematic new frontiers, that dividing North and South Vietnam, flared up immediately after he had taken office, forcing him to bolster the US-backed regime in Saigon. Kennedy examined "tricks and gadgets" that might give the South an edge in the jungle, and in November 1961 sanctioned the use of defoliants in a covert operation code-named Ranch Hand, every mission flown signed off by the president himself and managed in Saigon by the secret Committee 202 - the call sign for defoliating forests being "20" and for spraying fields "2".

Ngo Luc, 67, was serving with a North Vietnamese guerrilla unit in the Central Highlands when he saw planes circling overhead. "We expected bombs, but a fine yellow mist descended, covering absolutely everything," he says. "We were soaked in it, but it didn't worry us, as it smelled good. We continued to crawl through the jungle. The next day the leaves wilted and within a week the jungle was bald. We felt just fine at the time." Today, the former captain is the sole survivor from his unit and lives with his two granddaughters, both born partially paralysed, near the central Vietnamese city of Hue.

When US troops became directly embroiled in Vietnam in 1964, the Pentagon signed contracts worth $57m (£36m) with eight US chemical companies to produce defoliants, including Agent Orange, named after the coloured band painted around the barrels in which it was shipped. The US would target the Ho Chi Minh trail - Viet Cong supply lines made invisible by the jungle canopy along the border with Laos - as well as the heavily wooded Demilitarised Zone (DMZ) that separated the North from the South, and also the Mekong Delta, a maze of overgrown swamps and inlets that was a haven for communist insurgents.

A reporter for the St Louis Dispatch witnessed a secret spraying mission and wrote that the US was dropping "poison". Congressman Robert Kastenmeier demanded that the president abandon "chemical warfare" because it tainted America's reputation. Instead, William Bundy, a presidential adviser, flatly denied that the herbicide used by America was a chemical weapon, and blamed communist propagandists for a distortion of the facts about the Ranch Hand operation. Only when the Federation of American Scientists warned that year that Vietnam was being used as a laboratory experiment did the rumours become irrefutable. More than 5,000 American scientists, including 17 Nobel laureates and 129 members of the Academy of Sciences, signed a petition against "chemical and biological weapons used in Vietnam".

Eight years after the military launched Operation Ranch Hand, scientists from the National Institute of Health warned that laboratory mice exposed to Agent Orange were giving birth to stillborn or deformed litters, a conclusion reinforced by research conducted by the US department of agriculture. These findings coincided with newspaper reports in Hanoi that blamed Agent Orange for a range of crippling conditions among troops and their families. Dr Le Ke Son, a young conscript in Hanoi during the war and now director of Vietnam's Agent Orange Victims Fund, recalls, "The government proposed that a line of runners carry blood and tissue samples from the front to Hanoi. But it was more than 500 miles and took two months, by which time the samples were spoiled. How could we make the research work?

There was no way to prove what we could see with our own eyes." In December 1969, President Nixon made a radical and controversial pledge that America would never use chemical weapons in a first strike. He made no mention of Vietnam or Agent Orange, and the US government continued dispatching supplies of herbicides to the South Vietnamese regime until 1974.

That year, Kiem was born in a one-room hut in Kim Doi, a village just outside Hue. For her mother, Nguyen, she should have been a consolation because her husband, a Viet Cong soldier, had been killed several months earlier. "The last time he came home, he told me about the spray, how his unit had been doused in a sweet-smelling mist and all the leaves had fallen from the trees," Nguyen says. It soon became obvious that Kiem was severely mentally and physically disabled. "She can eat, she can smile, she sits on the bed. That's it. I have barely left my home since my daughter was born."

By the time the war finally ended in 1975, more than 10% of Vietnam had been intensively sprayed with 72 million litres of chemicals, of which 66% was Agent Orange, laced with its super-strain of toxic TCCD. But even these figures, contained in recently declassified US military records, vastly underestimate the true scale of the spraying. In confidential statements made to US scientists, former Ranch Hand pilots allege that, in addition to the recorded missions, there were 26,000 aborted operations during which 260,000 gallons of herbicide were dumped. US military regulations required all spray planes or helicopters to return to base empty and one pilot, formerly stationed at Bien Hoa air base between 1968 and 1969, claims that he jettisoned his chemical load into the Long Binh reservoir.

"These herbicides should never have been used in the way that they were used," says the pilot, who has asked not to be identified. Almost immediately after the war finished, US veterans began reporting chronic conditions, skin disorders, asthma, cancers, gastrointestinal diseases. Their babies were born limbless or with Down's syndrome and spina bifida. But it would be three years before the US department of veterans' affairs reluctantly agreed to back a medical investigation, examining 300,000 former servicemen - only a fraction of those who had complained of being sick - with the government warning all participants that it was indemnified from lawsuits brought by them. When rumours began circulating that President Reagan had told scientists not to make "any link" between Agent Orange and the deteriorating health of veterans, the victims lost patience with their government and sued the defoliant manufacturers in an action that was finally settled out of court in 1984 for $180m (£115m).

It would take the intervention of the former commander of the US Navy in Vietnam, Admiral Elmo Zumwalt, for the government finally to admit that it had been aware of the potential dangers of the chemicals used in Vietnam from the start of Ranch Hand. The admiral's involvement stemmed from a deathbed pledge to his son, a patrol boat captain who contracted two forms of cancer that he believed had been caused by his exposure to Agent Orange.

Every day during the war, Captain Elmo Zumwalt Jr had swum in a river from which he had also eaten fish, in an area that was regularly sprayed with the herbicide. Two years after his son's death in 1988, Zumwalt used his leverage within the military establishment to compile a classified report, which he presented to the secretary of the department of veterans' affairs and which contained data linking Agent Orange to 28 life-threatening conditions, including bone cancer, skin cancer, brain cancer - in fact, almost every cancer known to man - in addition to chronic skin disorders, birth defects, gastrointestinal diseases and neurological defects.

Zumwalt also uncovered irrefutable evidence that the US military had dispensed "Agent Orange in concentrations six to 25 times the suggested rate" and that "4.2m US soldiers could have made transient or significant contact with the herbicides because of Operation Ranch Hand". This speculative figure is twice the official estimate of US veterans who may have been contaminated with TCCD.

Most damning and politically sensitive of all is a letter, obtained by Zumwalt, from Dr James Clary, a military scientist who designed the spray tanks for Ranch Hand. Writing in 1988 to a member of Congress investigating Agent Orange, Clary admitted: "When we initiated the herbicide programme in the 1960s, we were aware of the potential for damage due to dioxin contamination in the herbicide. We were even aware that the military formulation had a higher dioxin concentration than the civilian version, due to the lower cost and speed of manufacture. However, because the material was to be used on the enemy, none of us were overly concerned."

The Office of Genetic Counselling and Disabled Children (OGCDC) operates out of a room little bigger than a broom cupboard. Dr Viet Nhan and his 21 volunteers share their cramped quarters at Hue Medical College with cerebral spinal fluid shunt kits donated from Norfolk, Virginia; children's clothes given by the Rotary Club of Osaka, Japan; second-hand computers scavenged from banks in Singapore.

Vietnam's chaotic and underfunded national health service cannot cope with the demands made upon it. The Vietnamese Red Cross has registered an estimated one million people disabled by Agent Orange, but has sufficient funds to help only one fifth of them, paying out an average of $5 (£3) a month. Dr Nhan established the free OGCDC, having studied the impact of Agent Orange as a student, to match Vietnamese families to foreign private financial donors. "It was only when I went out to the villages looking for case studies that I realised how many families were affected and how few could afford help," he says. "I abandoned my research. Children need to run before they die."

The walls of his room are plastered with bewildering photographs of those he has helped: operations for hernias and cleft palates, open-heart surgery and kidney transplants. All of the patients come from isolated districts in central Vietnam, villages whose names will be unfamiliar, unlike the locations that surround them: Khe Sanh, Hamburger Hill, Camp Carroll and the Rock Pile. "I am not interested in apportioning blame," Nhan says. "I don't want to talk to you about science or politics. What I care about is that I have 60 sick children needing financial backers. They cannot wait for the US to change its policy, take its head out of the sand and clear up the mess."

He takes us into an intensive care ward to meet nine-year-old Nguyen Van Tan, who two weeks before had open-heart surgery to correct a birth defect thought to be connected to dioxin poisoning. There is no hard proof of this, but his father, who sits beside the bed, talks of being sprayed with defoliants when he fought with the Viet Cong. The area they live in was repeatedly doused during the war. Almost all of his former battlefield comrades have disabled children, he says. Nhan ushers us away. "I don't want to tell the family yet, but their boy will never fully recover. He is already suffering from total paralysis. The most we can do now is send them home with a little money."

Back in his tiny office, the doctor gestures to photocopies of US Air Force maps, sent by a veterans' organisation because the US government refuses to supply them. These dizzying charts depict the number of herbicide missions carried out over Quang Tri, a province adjacent to the DMZ, from where almost all Nhan's patients come. Its topography is obliterated by spray lines, 741,143 gallons of chemicals dropped here, more than 600,000 of them being Agent Orange. "I'm just scratching the surface," he says.

The Vietnamese government is reluctant to let us travel to Quang Tri province. It does not want us "to poke and prod" already dismal villagers, treating them as if they are medical exhibits. We attempt to recruit some high-powered support and arrange a meeting in Hanoi with Madame Nguyen Thi Binh, who until last year was the vice-president of Vietnam. She receives us at the presidential palace in a teak-panelled hall beneath an enormous photograph of Ho Chi Minh in a gold frame writhing with dragons. "Thank you, my young friends, for your interest in Vietnam," Madame Binh says, straightening her grey silk ao dai, a traditional flowing trouser suit.

She looks genteel, but old photographs of her in olive fatigues suggest she is a seasoned campaigner. As minister of foreign affairs for the Provisional Revolutionary South Vietnamese government, she negotiated at the Paris peace talks in 1973. "I must warn you, I will not answer questions about George W Bush," she says, casting a steely gaze, perhaps conscious of the fact that, since the lifting of the US economic embargo in 1994, trade with America has grown to £650m a year.

Madame Binh does, however, want to talk about chemical warfare, recalling how, when she returned after the war to her home province of Quang Nam, a lush region south-west of Hue which was drenched in defoliants, she found "no sign of life, just rubble and grass". She says: "All of our returning veterans had a burning desire for children to repopulate our devastated country. When the first child was born with a birth defect, they tried again and again. So many families now have four or five disabled children, raising them without any hope."

What should the US do? Madame Binh laughs. "It's very late to do anything. We put this issue directly on the table with the US. So far they have not dealt with the problem. If our relationship is ever to be normal, the US has to accept responsibility. Go and see the situation for yourself." She sends us back to Hue. Over chilled water and tangerines, we talk to a suspicious party secretary who asks us why we have bothered to come after all these years. "There is no point," he says. "Nothing will come of it."

But he opens his file all the same and reads aloud: "In Hue city there are 6,633 households affected by Agent Orange and in them 3,708 sick children under the age of 16." He eventually agrees to take us north-west, over the Perfume river, beyond the ancient royal tombs that circle this former imperial city, towards the DMZ. We arrive at a distant commune where a handyman is sprucing up a bust of Ho Chi Minh with white gloss paint.

Eventually, the chairman of the People's Committee of Dang Ha joins us, and our political charabanc stuffed with seven officials sets out across the green and gold countryside, along crisscrossing lanes. The chairman tells us proudly how he was born on January 31 1968, the night of the Tet offensive, the turning point of the war, when the Viet Cong launched its assault on US positions. By the time we stop, we are all the best of friends and, holding hands, he pulls us into the home of the Pham family, where a wall of neighbours and an assembly of local dignitaries dressed in shiny, double-breasted jackets stare grimly at a moaning child.

He lies on a mat on the floor, his matchstick limbs folded uselessly before him, his parents taking it in turns to mop his mouth, as if without them he would drown in his own saliva. Hoi, the boy's mother, tells us how she met her husband when they were assigned to the same Viet Cong unit in which they fought together for 10 years. But she alone was ordered to the battle of Troung Hon mountain. "I saw this powder falling from the sky," she says. "I felt sick, had a headache. I was sent to a field hospital. I was close to the gates of hell. By the time I was discharged, I had lost the strength in my legs and they have never fully recovered. Then Ky was born, our son, with yellow skin.

Every year his problems get worse." Her husband, Hung, interrupts: "Sometimes, we have been so desperate for money that we have begged in the local market. I do not think you can imagine the humiliation of that." And this family is not alone. All the adults here, cycling past us or strolling along the dykes, are suffering from skin lesions and goitres that cling to necks like sagging balloons. The women spontaneously abort or give birth to genderless squabs that horrify even the most experienced midwives.

In a yard, Nguyen, a neighbour's child, stares into space. He has a hydrocephalic head as large as a melon. Two houses down, Tan has distended eyes that bubble from his face. By the river, Ngoc is sleeping, so wan he resembles a pressed flower. "They told me the boy is depressed," his exhausted father tells us. "Of course he's depressed. He lives with disease and death."

This is not a specially constructed ghetto used to wage a propaganda war against imperialism. The Socialist Republic of Vietnam has long embraced the free market. This is an ordinary hamlet where, in these new liberal times, villagers like to argue about the English Premiership football results over a glass of home-brewed rice beer. Here live three generations affected by Agent Orange: veterans who were sprayed during the war and their successors who inherited the contamination or who still farm on land that was sprayed. Vietnam's impoverished scientific community is now trying to determine if there will be a fourth generation. "How long will this go on?" asks Dr Tran Manh Hung, the ministry of health's leading researcher.

Dr Hung is now working with a team of Canadian environmental scientists, Hatfield Consultants, and they have made an alarming discovery. In the Aluoi Valley, adjacent to the Ho Chi Minh trail, once home to three US Special Forces bases, a region where Agent Orange was both stored and sprayed, the scientists' analysis has shown that, rather than naturally disperse, the dioxin has remained in the ground in concentrations 100 times above the safety levels for agricultural land in Canada.

It has spread into Aluoi's ponds, rivers and irrigation supplies, from where it has passed into the food chain, through fish and freshwater shellfish, chicken and ducks that store TCCD in fatty tissue. Samples of human blood and breast milk reveal that villagers have ingested the invisible toxin and that pregnant women pass it through the placenta to the foetus and then through their breast milk, doubly infecting newborn babies. Is it, then, a coincidence that in this minuscule region of Vietnam, more than 15,000 children and adults have already been registered as suffering from the usual array of chronic conditions?

"We theorise that the Aluoi Valley is a microcosm of the country, where numerous reservoirs of TCCD still exist in the soil of former US military installations," says Dr Wayne Dwernychuk, vice-president of Hatfield Consultants. There may be as many as 50 of these "hot spots", including one at the former US military base of Bien Hoa, where, according to declassified defence department documents, US forces spilled 7,500 gallons of Agent Orange on March 1 1970. Dr Arnold Schecter, a leading expert in dioxin contamination in the US, sampled the soil there and found it to contain TCCD levels that were 180 million times above the safe level set by the US environmental protection agency.

It is extremely difficult to decontaminate humans or the soil. A World Health Organisation briefing paper warns: "Once TCCD has entered the body it is there to stay due to its uncanny ability to dissolve in fats and to its rock solid chemical stability." At Aluoi, the researchers recommended the immediate evacuation of the worst affected villages, but to be certain of containing this hot spot, the WHO also recommends searing the land with temperatures of more than 1,000C, or encasing it in concrete before treating it chemically.

At home, the US takes heed. When a dump at the Robins Air Force Base in Georgia was found to have stored Agent Orange, it was placed on a National Priority List, immediately capped in five feet of clay and sand, and has since been the subject of seven investigations. Dioxin is now also a major domestic concern, scientists having discovered that it is a by-product of many ordinary industrial processes, including smelting, the bleaching of paper pulp and solid waste incineration. The US environmental protection agency, pressed into a 12-year inquiry, recently concluded that it is a "class-1 human carcinogen".

The evidence is categoric. Last April, a conference at Yale University attended by the world's leading environmental scientists, who reviewed the latest research, concluded that in Vietnam the US had conducted the "largest chemical warfare campaign in history". And yet no money is forthcoming, no aid in kind. For the US, there has only ever been one contemporary incident of note involving weapons of mass destruction -Colin Powell told the UN Security Council in February that, "in the history of chemical warfare, no country has had more battlefield experience with chemical weapons since world war one than Saddam Hussein's Iraq".

The US government has yet to respond to the Hatfield Consultants' report, which finally explains why the Vietnamese are still dying so many years after the war is over, but, last March, it did make its first contribution to the debate in Vietnam. It signed an agreement with a reluctant Vietnamese government for an $850,000 (£543,000) programme to "fill identified data gaps" in the study of Agent Orange. The conference in Hanoi that announced the decision, according to Vietnamese Red Cross representatives who attended, ate up a large slice of this funding. One of the signatories is the same US environmental protection agency that has already concluded that dioxin causes cancer.

"Studies can be proposed until hell freezes over," says Dr Dwernychuk of Hatfield Consultants, "but they are not going to assist the Vietnamese in a humanitarian sense one iota. We state emphatically that no additional research on human health is required to facilitate intervention or to protect the local citizens."

There is cash to be lavished in Vietnam when the US government sees it as politically expedient. Over the past 10 years, more than $350m (£223m) has been spent on chasing ghosts. In 1992, the US launched the Joint Task Force-Full Accounting to locate 2,267 servicemen thought to be missing in action in Vietnam, Cambodia and Laos. Jerry O'Hara, spokesman for JTF-FA, which is still searching for the remains of 1,889 of them, told us, "We don't place a monetary value on what we do and we'll be here until we have brought all of the boys back home."

So it is that America continues to spend considerably more on the dead than it does on the millions of living and long-suffering - be they back home or in Vietnam. The science of chemical warfare fills a silent, white-tiled room at Tu Du hospital in Ho Chi Minh City. Here, shelves are overburdened with research materials. Behind the locked door is an iridescent wall of the mutated and misshapen, hundreds of bell jars and vacuum-sealed bottles in which human foetuses float in formaldehyde. Some appear to be sleeping, fingers curling their hair, thumbs pressing at their lips, while others with multiple heads and mangled limbs are listless and slumped. Thankfully, none of these dioxin babies ever woke up.

One floor below, it is never quiet. Here are those who have survived the misery of their births, ravaged infants whom no one has the ability to understand, babies so traumatised by their own disabilities, luckless children so enraged and depressed at their miserable fate, that they are tied to their beds just to keep them safe from harm.